抗 PLXDC2 ウサギモノクローナル抗体


細胞表面1回膜貫通タンパク質であるPlexin domain containing 2 (PLXDC2) は、造血幹細胞、神経幹細胞、多能性幹細胞および癌細胞を含む多くの組織で発現している。PLXDC2 は、神経細胞の成長、幹細胞の発達、血管新生および癌細胞の増殖において重要な役割を果たしている。PLXDC2は、脳卒中患者の末梢血、ヘリコバクター ピロリに曝露されたマウス骨髄由来マクロファージ で発現上昇が報告されている。アカゲザルラジノウイルス(RRV)の受容体としての役割やアルツハイマー病に関わるプロテアーゼ BACE1 (β-Site APP Cleaving Enzyme)によって、アミロイド前駆体タンパク質等と共に細胞外ドメインが切断されることも知られている。

Plexin domain containing protein 2 (PLXDC2), a cell surface transmembrane protein, is expressed in many tissues including haemotopoeitc stem cells, neural stem cells, pluripotent stem cells, and tumor cells. PLXDC2 may play an important role in neuronal growth, stem cell development, angiogenesis, and cancer cell growth. Recently PLXDC2 was reported a good cell surface marker as human haemotopoetic stem cells (1).

PLXDC2 has been reported as a receptor for pigment epithelium derived factor (PEDF) (2) or as an activating ligand for adhesion G-protein coupled receptor D1 (Adgrd1) (3). Also PLXDC2 was thought as a novel interaction partner and an entry receptor for rhesus monkey rhadinovirus (RRV) (4). The gene expression level of PLXDC2 was elevated in the peripheral blood of stroke patients (5) or in mouse bone marrow-derived macrophages in response to Helicobacter pylori (6). The protease BACE1 (β-Site APP Cleaving Enzyme), a major drug target in Alzheimer’s disease, cleaves the amyloid precursor protein (APP) as well as PLXDC2 as one of several other substrates (7).

(1) Tanaka Y et al 2021 bioRXiv Sep 27: 2021.09.27.461900v1.doi: 10.1101/2021.09.27.461900.
(2) Cheng G et al. 2014 Elife. 3:e05401
(3) Bianchi E et al 2021 Nat Commun. 2021 12(1):1251.
(4) Großkopf AK et al. 2021 PLoS Pathog. 17(3):e1008979
(5) O’Connell GC et al. 2017 Genom Data. Sep 14:47-52.
(6) Tubau-Juni N, et al 2020 Sci. Rep. 10(1):11506.
(7) Dislich B et al. 2015 Cell Proteomics. 10:2550-63



  抗 PLXDC2 ウサギモノクローナル抗体一覧



  1. Shimizu, H. et al. Improving the quality of a recombinant rabbit monoclonal antibody against PLXDC2 by optimizing transient expression conditions and purification method. Protein Expr. Purif. 146, 27–33 (2018).
  2. Yamamoto N. et al.Expression Pattern of Plexin Domain Containing 2 in Human Hepatocellular Carcinoma. Monoclon Antib Immunodiagn Immunother. 39(2):57-60 (2020)
  3. Hamada, Y. et al. Plexin domain containing protein 2 is more expressed within the invasive area of human colorectal cancer tissues. Human Cell 34(5) 1580-1583 (2021).
  4. Tanaka Y. et al. Prospective isolation of mouse and human hematopoetic stem cells using Plexin domain containing 2. bioRXiv Sep 27: 2021.09.27.461900v1.doi: 10.1101/2021.09.27.461900.
  5. Akuta T. et al. A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting. Antibodies (Basel). 2022 May 12;11(2):36. doi: 10.3390/antib11020036.
  6. Sakuma C. et al. Western blotting of native proteins from agarose gels. Biotechniques. 2022 May;72(5):207-218. doi: 10.2144/btn-2022-0012.